- Josuke Vs. Joshu Higashikata
- Josuke Vs. Ojirou Sasame
- Josuke Vs. Daiya Higashikata
- Josuke & Paisley Park Vs. Kyo Nijimura
- Josuke Vs. The population of Shakedown Road
- Josuke & Norisuke Higashikata IV Vs. Yotsuyu Yagiyama
- Josuke Vs. Jobin Higashikata
- Josuke & Karera Sakunami Vs. A. Phex Brothers
Josuke makes his debut in the PS3 game, as the only Part 8 playable character. He was confirmed for the game alongside the first DLC character, Yoshikage Kira.
Josuke's appearance in the game was a surprise for fans, as Part 8 was still fairly recent and only 3 volumes into its story. As a result most of his moves, animations, taunts and such are based on those three volumes, along with a few liberties, making a couple of his animations somewhat limited or even awkward.
With the absence of a major antagonist and main plot at the time, Part 8's Story Mode consists of Josuke facing all the previous JoJo protagonists (starting with Jonathan all the way to himself) with no logical reason. They all appear from the Wall Eyes like Josuke and see him as an enemy, sinking back into the ground when defeated. Baoh also appears as the last Extra Story fight with Josuke, with their battle named "Baoh the Visitor". After defeating them all, Josuke proceeds to search for Yasuho Hirose before the "To Be Continued" arrow appears, putting in motion the story of JoJolion.
As one of the mass majority of playable characters in the game with the "Stand" Style, Josuke can turn Soft & Wet on/off, changing movesets, as well as having access to the Stand Rush ability returning from the Capcom game, being able to attack in conjunction with his Stand.
- Throw - I've taken your friction: Soft & Wet takes the friction from the opponent's feet, making them slip and fall as Josuke knees them in the face when they come down.
While Soft & Wet is off:
- Quit screwing around!: Josuke stomps. This skill can hit a downed opponent, and all of Josuke's moves can be performed instantly following it. (Comboable)
- So slippery!: Josuke removes his own friction as he spins around on the floor, hitting the opponent multiple times before getting back up. This move is a low attack and cannot be blocked if the opponent is not crouching. Before Josuke gets back up, a follow-up move can be used. (Comboable through Puttsun Cancel)
- Zero Friction: Josuke stops spinning to slide forward and unleash a double kick that knocks the opponent off their feet. This move is a low attack and cannot be blocked if the opponent is not crouching. (Comboable through Puttsun Cancel)
- Let me take that from you.: Josuke throws out bubbles which slowly float forward as projectiles before popping. (Comboable)
- Don't mess with me!: Josuke slides forward in a crouch to deliver a potent punch to the opponent's lower body, leaving them crumpling. (Comboable)
- How to use the bubbles: Josuke surrounds himself with bubbles as he shaves. The bubbles act as a trap and obstacle, damaging the opponent if they come in contact, though easily popped by most other projectiles. (Comboable)
While Soft & Wet is on:
- Don't get any ideas!: Josuke and Soft & Wet leap forward and fly through the air as S&W rapidly punches (Comboable through Puttsun Cancel). S&W finishes by grabbing the opponent and throwing them behind.
- The hell is your problem?!: Josuke jumps backward into a crouch as Soft & Wet advances forward while punching. This ability can initiate Rush Mode. A Stand Rush skill that Josuke can disconnect from to attack the opponent while it is executing. (Comboable through Puttsun Cancel)
- I gotta win this!: Soft & Wet delivers a powerful roundhouse kick that sends the opponent into the stage wall. A Stand Rush skill that Josuke can disconnect from to attack the opponent while it is executing. (Comboable through Puttsun Cancel)
Josuke's HHA, "I can steal your eyesight.", has him send out a fast-moving bubble created by Soft & Wet. If it hits the opponent, the camera shifts to the opponent's point of view as the bubble floats up to their eyes and pops, stealing their vision and leaving them crumpling (recreating Josuke's self-defense against Joshu in the first chapter of Part 8). Since the HHA results in a soft knockdown (assuming the opponent is on the ground when hit), Josuke can follow up on it if quick enough, either with a second HHA (resulting in a hard knockdown), or even his GHA, marking it the only HHA in the game that doesn't end a combo on success.
Josuke's GHA, simply named "Soft & Wet", has him pull out a homing bubble from his Joestar Birthmark and fling it. If it hits the opponent, their body's friction is taken, making them slip to the ground and be left vulnerable. As Josuke calmly and confidently explains what happened to them, Soft & Wet delivers an uppercut that knocks them into the air, before unleashing upward barrage of punches, finishing with a powerful delayed hook that sends the opponent spinning out.
Josuke has an alternate costume based on the attire from the first image of him ever released (the one used to confirm and promote Part 8 in Ultra Jump). His main color scheme is based on the same image, while his alternate color, which is white, appears to reference Part 8's Yoshikage Kira, as when Soft & Wet is activated, it is pink in coloration similar to that traditionally given to Killer Queen.
For the western version of the game, Josuke was named "Josuke Higashikata 8" to avoid confusion with Part 4's Josuke Higashikata, who was named "Josuke Higashikata 4".
He forms a tag-team with Joshu as the only two Part 8 characters in the game.
He is paired with Narciso Anasui in the Eyes of Heaven Tournament, having been placed in the F Block series of battles to be incorporated into the main Tournament. They were eliminated in the first round by Part 4 Jotaro Kujo and Diego Brando from Another Universe.
- When he is given the name "Josuke Higashikata", his name is written in a different kanji (定助 instead of 仗助) than that of the Part 4 protagonist's (of note is that this name is pronounced the same as Norisuke IV's birth name).
- The brand of his hat is "SBR" (a reference to the Steel Ball Run race).
- Josuke was originally going to have the Stand ability to attach screws to something, but Araki changed his mind and gave that to Joshu. 
- Fans gave him the name Jo2uke or Gappy (in reference to the distinct gap in his teeth) to avoid confusion with the other Josuke.