DIO's Curse (DIOの呪縛 DIO no Jubaku) is the twenty-ninth episode of the JoJo's Bizarre Adventure anime and the third episode of Stardust Crusaders. It covers Chapter 120 through half of Chapter 122 of the manga.
According to Joseph, the flesh bud attached to Kakyoin's brain allowed DIO to force allegiance from him through charisma. Avdol recalls having almost fell victim to one back in Egypt where he met DIO, though was fortunate enough to escape. Despite the odds, Jotaro takes it upon himself to surgically remove the bud from Kakyoin's head, claiming that Star Platinum's sharp precision would give him an advantage. Jotaro successfully the removes the bud after some effort, and Joseph finishes it off with the Ripple.
The next day, as Avdol walks to the kitchen, he stumbles upon a spoon on the ground, and soon after Holy's unconscious body slumped in front of the refrigerator. He discovers translucent rose vines growing from her backside and concludes it to be a Stand. Though due to Holy's kind nature, she lacked the willpower to manifest it properly and it was slowly killing her instead. Jotaro and Joseph arrive at the scene, where Joseph acts out in rage having his worst fears assured. Taking the initiative, Jotaro asks for a way to save her, but apparently the only way to do so was to kill DIO. Using a photo previously taken with Hermit Purple, Jotaro uses Star Platinum to identify a fly lingering in the darkness behind DIO. Avdol recognizes this fly and determines it to be of a certain species found only in the Aswan region of Egypt, thus leading them to assume DIO was in that location. Kakyoin appears and confirms their suspicions, stating that he had met DIO in that area on a family vacation. With approximately 50 days before Holy dies, Joseph calls in the Speedwagon Foundation to watch over her while the group sets off for Egypt by plane.
In another location, DIO uses a power similar to Hermit Purple to take a photograph of the Joestar group. Joseph becomes cautious after sensing them being watched and tells Jotaro to stay alert for any trouble. At that point, a small insect flies into the open.
- In the manga, Avdol discusses Holy's condition with Kakyoin outside after discovering DIO's location. In the anime, he does so while searching for the encyclopedia in the library.
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